|
KMID : 0380620070390040464
|
|
Korean Journal of Food Science and Technology
2007 Volume.39 No. 4 p.464 ~ p.469
|
|
|
In vitro Anti-inflammatory Activity of the Artemisia fukudo Extracts in Murine Macrophage RAW 264.7 Cells
|
|
Yoon Weon-Jong
Lee Jung-A
Kim Kil-Nam
Kim Ji-Young
Park Soo-Yeong
|
|
|
Abstract
|
|
|
|
The present study describes the preliminary evaluation of the anti-inflammatory activities of Artemisia fukudo extracts. The 80% ethanol extract of A. fukudo was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. In order to effectively screen for anti-inflammatory agents, we first examined the extracts` inhibitory effects on the production of pro-inflammatory cytokines activated with lipopolysaccharide. Moreover, we examined the inhibitory effects of the A. fukudo extracts on pro-inflammatory factors (NO, iNOS, COX-2, and PGE2) in murine macrophage RAW 264.7 cells. The protein levels were determined by immunoblotting. Of the sequential solvent fractions, the n-hexane and dichloromethane fractions inhibited the mRNA expression of pro-inflammatory cytokines (TNF-a, IL-1β, and IL-6), production of NO and PGE2, and the protein levels of iNOS and COX-2. These results suggest that A. fukudo may have significant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic plant.
|
|
|
KEYWORD
|
|
|
Artemisia fukudo, pro-inflammatory cytokines, NO, PGE2, iNOS, COX-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|